The rabbit anti-rat brain associated 0(RBAO) serum wich was obtained by immunization of rabbit with DA rat brain tested against rat lymphoid tissues for cytotoxicity, indirect immunofluorescent staining and ability to inhibit a graft-vs.-host reaction.. It was founded that the antiserum was a potent anti-0 like antiserum, and rat brain associated 0 antigen was cross-reactive with mouse thymocytes and brain antigen.
Using the RBA 0 sera, distribution of 0-bearing lymphocytes in rat lymphoid tissues was detected. And it was found that approximately 93% of thymocytes, 70-76% of lymph node lymphocytes, 72% of peripheral blood lymphocytes, 36-44% of spleen lymphocytes, and 4% of bone marrow were -bearing.
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